store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sulfo-NHS-SS-Biotin sodium (Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate) 是一种长链可裂解和细胞无渗透的胺类活性生物素化 (biotinylation) 试剂。Sulfo-NHS-SS-Biotin sodium 可用于细胞表面蛋白 (cell-surface protein) 的标记和纯化。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 777 | 待询 | ||
50 mg | ¥ 2,398 | 待询 | ||
100 mg | ¥ 3,795 | 待询 |
产品描述 | Sulfo-NHS-SS-Biotin sodium (Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate) is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent. It can be used for the labeling and purifying of cell-surface protein. |
靶点活性 | Cell-surface protein: |
体外活性 | Sulfo-NHS-SS-biotin is a negatively charged reagent and does not permeate cell membranes. Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides[1]. Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail [2]. |
别名 | Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate, Sulfo-NThiol-SS-biotin, Sulfo-NHS-SS-Biotin (sodium), Sulfo-NHS-SS-Biotin |
分子量 | 606.69 |
分子式 | C19H27N4NaO9S4 |
CAS No. | 325143-98-4 |
store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 180 mg/mL (296.7 mM), Sonication is recommended.
DMSO: 100 mg/mL (164.8 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O / DMSO | 1 mM | 1.6483 mL | 8.2414 mL | 16.4829 mL | 41.2072 mL |
5 mM | 0.3297 mL | 1.6483 mL | 3.2966 mL | 8.2414 mL | |
10 mM | 0.1648 mL | 0.8241 mL | 1.6483 mL | 4.1207 mL | |
20 mM | 0.0824 mL | 0.4121 mL | 0.8241 mL | 2.0604 mL | |
50 mM | 0.033 mL | 0.1648 mL | 0.3297 mL | 0.8241 mL | |
100 mM | 0.0165 mL | 0.0824 mL | 0.1648 mL | 0.4121 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Sulfo-NHS-SS-Biotin sodium 325143-98-4 Others Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate Sulfo-NThiol-SS-biotin Sulfo-NHS-SS-Biotin (sodium) Sulfo-NHS-SS-Biotin Inhibitor inhibitor inhibit